Abstract:Objective To analyse the clinical significance of the immunophenotype, aneuploidy and cell circle distribution in children with acute leukemia (AL). Methods With the techniques of flow cytometry (FCM), the immunophenotype and the DNA content in bone marrow mononuclear cells were determined in 42 cases of confirmed AL patients. Results The expression frequency of myeloid antigens in the ANLL group was CD 33 >CD 13 >CD 14 >CD 15 . Both lymphoid and myeloid antigen were expressed in the 4 AL patients with poor prognosis. The percentage of myelocytic circles (S%) in AL patients was significantly lower than in normal controls, indicating hemopoietic hypofunction in AL patients. The S% medium of hypodiploidy in the ALL group was significantly different compared with that of hypodiploidy and diploidy in the ALL group. The S% medium in patients with CD 33 /CD 13 >1 was marked different compared with those with CD 33 /CD 13 <1 in the ANLL group. Conclusions The analysis of immunophenotype, aneuploidy, and cell circle plays an important role in clinical diagnosis, treatment and prognosis.
