Abstract:Objective To study the changes of TXB2 and 6 - keto - PGF1α in the, lung of immature rabbits with meconium aspiration. Methods We established mild and severe immature rabbit models of meconium aspiration by endotracheal intubation imbuing meconium 0. 6 ml/kg and 4 ml/kg. We measured the right ventricular pressure by right ventricular puncture. Levels of TXB2 and 6 - keto - PGF1α in the lung were measured by radioimmunoassay. Results In the mild group, the right ventricular pressure began to increase at 16 hours (2. 57 ± 0. 10 kPa), peaked at 24 hours (3. 57 ± 0. 14 kpa) and returned to the normal level by 72 hours (1. 89 ± 0. 04 kPa). The pressure of the severe group (4. 36 ± 0. 14 kpa)was significantly higher than that of the control (1. 85 ± 0. 05 kPa) and mild groups. Levels of TXB2 and 6 - keto - PGF1α. in the lung of the mild group increased significantly compared with the control group (76. 40 ± 16. 89 pg/ml; 56. 10 ± 16. 46 pg/ml), began to increase at 16 hours (153. 80 ± 15. 37 pg/ml, 117. 40 ± 22. 88 pg/ml),. peaked at 24 hours (369. 00 ± 28. 80 pg/ml; 207. 20 ± 28. 59 pg/ml) and recovered by 72 hours (103. 20 ± 11. 95 pg/ ml, 83. 62 ± 8. 76 pg/ml). The changes of the severe group (500. 60 ± 24. 58 pg/ml; 300. 00 ± 20. 31 pg/ml) were significantly different from those in the control and mild groups. The level of TXB2 and 6 - keto - PGF1. were correlated with the right ventricular pressure (r = 0. 95: 0. 96. P <0. 01). Conclusions TXB2 and 6 - keto -PGF1α in the lung significantly increase following the severity of meconium aspiration, indicating that TXB2 and 6 - keto - PGF1. influence the development of lung injury of meconium aspiration.
