Abstract:OBJECTIVE: S100A4 gene plays an important role in neuroblastoma cell invasion and metastasis. This paper aimed to evaluate the liposome-mediated transfection efficacy of S100A4 antisense oligodeoxynucleotide (AS-ODN) and its stability in neuroblastoma cells. METHODS: The fluorescence (FAM) labeled S100A4 AS-ODN was transfected with Lipofectamine~(TM) 2000 into human neuroblastoma cells (LA-N-5). The transfection efficacy, stability and persistence time were observed by laser scanning confocal microscopy, and were compared with those of naked AS-ODN without Lipofectamine~(TM) 2000. RESULTS: The transfection efficacy of the S100A4 AS-ODN mediated by Lipofectamine~(TM) 2000 in neuroblastoma cells was high.The maximal signal intensity of intracellular fluorescence was 173 at 8 hrs after transfection and remained as high as 135 at 24 hrs. The maximal efficacy of transfection was 68%, and the stable expression lasted for more than 72 hrs. The transfection efficacy of the naked AS-ODN without Lipofectamine~(TM) 2000 was 35%. The maximal signal intensity of intracellular fluorescence was 90 and occurred at 6 hrs after transfection. The stable expression lasted for only 12 hrs. CONCLUSIONS: The S100A4 AS-ODN can be effectively transfected with Lipofectamine~(TM) 2000 into neuroblastoma cells, and be stably expressed,which may be useful in gene therapy for neuroblastoma
