Abstract:Objective To determine the protective effects of Danshen complex injection against hypoxia-mediated injury in myocaridium.Methods Twenty-five mice were divided into normal control (CON) , hypoxia (HYP) , and Danshen (DAN) treatment groups. Myocardium samples were taken at two weeks after the start of experimental challenge. Myocardium succinic dehyrogenase (SDH), adenosine triphosphatase (ATP) and lactic dehydrogenase (LDH) were measured. Pathologic changes of myocardium were observed under the light and electric microscopes.Results In HYP, SDH and ATP drecrease and LDH increase were observed ; in addition, the mesenchyma of the myocardium was edematous and mitochondrions of myocardial cells were enlarged ; the arragement of filaments was irregular. However, there was no significant difference in myocardium enzyme levels and morphological structure between DAN and CON groups.Conclusions Enzymohistochemical and morphological findings in this study suggest that hypoxia causes myocardium impairment in mice and that Danshen can protect myocardium against hypoxia-induced injury.
