Abstract:Objective To study the value of fecal calprotectin(FC) in the diagnosis of neonatal necrotizing enterocolitis(NEC) through a Meta analysis. Methods Web of Science, Cochrane Library, PubMed, Embase, China National Knowledge Infrastructure, Weipu Periodical Database, Wanfang Data, Chinese Biomedical Literature Database were searched for related studies published up to May 2020, with manual search as supplementation. The QUADAS criteria were used to evaluate the quality of the articles included. Meta-DiSc 1.4 and Stata 15.0 software were used to perform the Meta analysis, including the evaluation of specificity, sensitivity, likelihood ratio, and diagnostic odds ratio. The sensitivity analysis and heterogeneity testing were performed, and the summary receiver operating characteristic(SROC) curve and Fagan diagram were plotted. Results A total of 15 articles were enrolled, involving 1 719 neonates. Among these articles, 4 had low quality, 2 had high quality, and the rest had medium quality. There was high heterogeneity between studies, and there was no threshold effect or publication bias. The random effects model analysis showed that FC had a pooled specificity of 0.80(95%CI: 0.78-0.82) and a sensitivity of 0.86(95%CI: 0.83-0.89) in the diagnosis of NEC, with a negative likelihood ratio of 0.19(95%CI: 0.14-0.26), a positive likelihood ratio of 4.71(95%CI: 3.57-6.23), and a diagnostic odds ratio of 29.56(95%CI: 17.98-48.61). The area under the SROC curve was 0.9131 and the Q* index was 0.8456. The Fagan diagram showed that the post-test probability of NEC indicated by negative FC was 13%, while that indicated by positive FC was 86%. The Meta regression analysis showed that the heterogeneity came from other non-threshold factors. Conclusions FC has high potential and efficiency in the early diagnosis of NEC. FC measurement can be used for the diagnosis of NEC, but it should be combined with clinical manifestations and other related laboratory examinations.
