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Abstract:Abstract: OBJECTIVE: To study the TH2 state as represented by the percentage of CD30+ cells, levels of IL24, IL25, and IL26 in PBMC, and serum IgE levels in children with the steroid sensitive nephrotic syndrome(SSNS). METHODS: CD30+ cells were assayed using FACS. IL24, 5, 6 and serum IgE were determined using EL ISA. RESULTS: There was a higher percentage of CD30+ cells (11.96%) and higher levels of IL24 (lg2.11 pg/ml) and serum IgE (299 IU/ml) in children with SSNS compared to the healthy control group (2.81%, lg1.37 pg/ml, 110 IU/ml, respectively) (P<0.01). No significant difference was found in the levels of IL25 and IL26 in the two groups. There was a significant correlation between the percentage of CD30+ cells and IL24(r=0.94, P<0.01) and between CD30+ cells and serum IgE(r=0.740 P<0.01). NO correlation was noted with IL25(r=-0.39, P>0.05)or with IL26 levels (r=0.08,P>0.05). CONCLUSIONS: The abnormal increase of CD30+ cells does not mean that all TH2 cells are activated. The TH1/TH2 imbalance in children with SSNS is not proved in this study.

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OBJECTIVE: To observe the variation of GH2IGF axis in children with the refractory nephrotic syndrome (RNS) . METHODS:  Serum and urine levels of IGF2I and IGFBP23 and baseline serum levels of GH were assayed using
RIA and IRMA in 26 patients with RNS , and hight standard deviation score (HtSDS) was calculated. Eighteen healthy children of similar ages were used as the control group (NC group) . RESULTS:　Serum IGF2I [ (152. 68 ±120. 95) ng/ ml ] and IGFBP23 [ (2 183. 33 ±1 711.33) ng/ ml ] levels in the RNS group were significantly lower than those of the NC group [ (255. 68 ±46. 92) ng/ ml, 4 333.87 ±1 122.00) ng/ ml ] (P<0.05), and urine IGF2I [ (5. 32 ±2. 84) ng/ mg creatinine ] and IGFBP23 [ (16. 38 ±8. 55) ng/ mg creatinine ] levels were higher than those of the NC group [ (0.90 ±0.37) ng/ mg creatinine, (5.13 ±1. 64) ng/ mg creatinine ] ( P<0.05). The serum GH level was lower than that of the NC group, but didn’t achieve any statistical significance. HtSDS (-0.42±0.75) of the RNS group was lower than that of the NC group (0.30±0.17) (P<0.05). CONCLUSIONS:　A disorder of the GH2IGF axis was detected in children with RNS. This abnormality may contribute to the growth failure seen in RNS.

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OBJECTIVE: To analyze the relationship between the clinical and pathological effects and longterm prognosis in children with Henoch Schonlein nephritis. METHODS: Changes of clinical pathology were studied in 32 children with Henoch Schonlein nephritis and 19 cases of them were followed over an 8 to 14 year period. RESULTS: Acute nephritis ranked first (50%) and the nephritic syndrome ranked second (40%) in the clinical classification of Henoch Schonlein nephritis; the majority had pathological changes of Grade Ⅱ～Ⅲ. The rate of recovery of acute nephritis and the nephritic syndrome was 55.6% and 28.6%, respectively. The rate of recovery and deterioration of Grade Ⅰ～Ⅲ pathological changes was 43.8% and 12.5%, respectively. Of the patients with Grade Ⅳ～Ⅴ pathological changes, 66.7% deteriorated or died. CONCLUSIONS: The prognosis of acute nephritis was better than that of the nephritic syndrome, and longterm prognosis is closely associated with the clinical classification and pathology

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OBJECTIVE: To investigate the therapeutic effect of Duoxikang capsula (DXK) on hyperlipoidemia in children with the steroid sensitive nephrotic syndrome (SSNS). METHODS: Twenty SSNS children were randomly divided into a DXK treated group and a control group. DXK was then administered (45 mg/kg weight daily) in the DXK treated group and the same regular steroid therapeutic measurements were conducted in two groups of SSNS. Bloodlipoid compliments were determined, including total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoproteincholesterol (LDL-C) before the randomization, and again 2～3 weeks, 5～6 weeks, 8～10 weeks after the treatment. RESULTS: During the 2～3 weeks, levels of LDL-C ［(3.19±2.08) mmol and TC ［(6.42±2.04) mmol］ in the DXKtreated group were markedly lower than those in the control group ［(5.82±2.73) mmol, (10.0±4.75) mmol, respectively］ (both P<0.05). During the 5～6 weeks and 8～10 weeks, the level of LDL-C in the DXK treated group was also lower than that in the control group ［(2.83±1.50) mmol vs (4.94±2.04) mmol; (2.30±0.46) mmol vs (4.20±2.22) mmol, respectively. P<0.01 . CONCLUSIONS: DXK treatment reduced severe hyperlipoidemia, especially LDL-C in children with SSNS. It is suggested that DXK treatment may reduce the secondary renal injury in these children.

Abstract:OBJECTIVE: To investigate the relationship between the disease and the personality and psychological status of children with the nephrotic syndrome (NS) or acute glomerulonephritis (AGN) and their parents. METHODS: We studied the personality and psychological profiles of the patients and their parents using the EPQ and SCL-90 inventories. RESULTS: In the NS group, the score of P.E inventory was lower while the score of N.L inventory was higher than that in the control group (P<0.05). However, the score of P.E.N.L inventory did not show any significant difference between the AGN group and the control group (P>0.05). The results of SCL-90 score showed that the NS group had higher scores in the somatization, interpersonal sensetivity, depression, anxiety, hostility, fear and paranoia (but not obsession and psychiatrics) sections of the test compared to the control group (P<0.05). However, there was no significant difference between the AGN group and the conrol group except in the somatization score. On the other hand, all scores of the parents of the patients were higher than those in the control group (P<0.05). CONCLUSIONS: Children with NS show a tendency towards introversion and emotional liability, and are more likely to suffer from depression, anxiety, fear and somatization. There was no significant difference between the AGN group and the control group in personality and psychology. The parents of children with NS or AGN have a tendency of introversion and neuoticism. They also have obvious symptoms of depression, anxiety, fear and somatization.

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OBJECTIVE: To study the relationship between the nephrotic syndrome(NS) and active infection with human cytomegalovirus (HCMV). METHODS: HCMV-DNA in peripheral leukocytes from 36 cases of NS was detected with the polymerase chain reaction (PCR) technique. HCMV-IgM antibody detection was done using ELISA simultaneously. RESULTS: The positive rates of active HCMV-IgM antibody and HCMV-DNA were 30.5% and 38.9%, respectively, higher than those of the control group (both P<0.01). HCMV-IgM and HCMV-DNA were found simultaneously positive in 8 cases. CONCLUSIONS: Some children with NS have HCMV infection.

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OBJECTIVE: To study the relationship between hyperlipidemia and pathologic renal changes in children with the primary nephrotic syndrome(NS). METHODS: Forty five children with no minimal change glomerulopathy (NMCD) (clinical type: steroidresistent NS) and 10 children with minimal change glomerulopathy (MCD) (clinical type: steroid sensitive NS) were compared with 80 healthy children. Seven lipoprotein metabolism parameters including serum total cholestero1 (TC), triglyceride (TG), highdensity lipoprotein cholesterol (HDL-C), lowdensity lipoprotein cholesterol (LDL-C), apolipoprotein AI (ApoAI), apolipoprotein B (ApoB) and lipoprotein (a) ［Lp (a)］ were detected using enzyme methods. RESULTS: After the treatment of the children with prednisone for 2 months, lipoprotein metabolism parameters remained significantly higher in the NMCD group compared to the controls: serum TC ［(6.54±4.33) mmol/L (NMCD) vs (3.94±0.67) mmol/L (control)］, TG ［(3.45±2.56) mmol/L vs (0.91±0.32) mmol/L］, HDL-C ［(1.62±0.79) mmol/L vs (1.31±0.32) mmol/L］, LDL-C［ (2.69±0.87) mmol/L vs (2.15±0.58) mmol/L］, ApoAI　［(1.51±0.54) g/L vs (1.30±0.58) g/L］, ApoB ［(1.45±0.54) g/L vs (0.67±0.16) g/L］, Lp(a) ［(360.6±179.4) g/L vs (162.5±128.5) g/L］ (P<0.05 or 0.01). In contrast, all abnormal lipoprotein metabolism parameters in the MCD cases recovered after prednisone treatment. CONCLUSIONS: There are obvious and long-term abnormialities of serum lipoprotein metabolism parameters in the NMCD group. NMCD cases should be treated with lipidlowering drugs early, while MCD cases should not be treated with lipid lowering drugs.

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OBJECTIVE: To review the etiology of fever of unknown origin (FUO) and the methods used to establish a diagnosis. METHODS: A retrospective review of the medical records of 317 patients with FUO admitted between January, 1996 and December, 2000 was performed. RESULTS: Of the 317 children, 298 cases (94.0%) had a definitive etiology established. Of the 298 cases, 160 (53.7%) had infectious diseases and non infectious diseases, such as collagen vascular disease, and neoplasm which accounted for a large fraction of the remainder. A diagnosis was established in 140 (47.0%) by comprehensive clinical analysis alone. Culture of bacteria in serum and biopsy specimens established a diagnosis in 64 cases (21.5%) and 37 cases (12.4%), respectively. Noninvasive imaging techniques (35 cases; 11.7%), autopsy (11 cases; 3.7%), bone marrow examination (6 cases; 1.9%) and retrospective diagnosis (5 cases; 1.7%) accounted for the remainder of the diagnosis. CONCLUSIONS: Most cases of FUO can be diagnosed by clinical characteristics of the patients and by essential laboratory studies. Pathological examination is very important in diagnosing the etiology of FUO. Infectious diseases, collagen vascular disease and neoplasm are the major causes of FUO in children.

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Abstract:OBJECTIVE: To determine whether the tetrapeptide N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP), a physiological hemorcgulatory inhibitor, has a biological function in nonhematopoietic cells (rat renal interstitial fibroblasts). METHODS: Cell proliferation was measured using standard cell culture techniques and the alama blue colorimctric assay. RESULTS: OD measurement of alamar blue absorption at 48 h was significantly inhibited in fibroblasts exposed to 1 μmol AcSDKP (P<0.01). During the 48hour alamar blue absorption, only AcSDKP in concentrations of 10-7 mol/L, 10-6 mol/L, and 10-5 mol/L had significant inhibiting effects compared to cells grown in the 5% FBS RPMI 1640 media alone. The inhibition was dose dependent, and disappeared at AcSDKP concentrations higher than 10-5 mol/L or lower than 10-8 mol/L. Maximum inhibition occurred at an AcSDKP concentration of 10-6 mol/L (33% suppression). CONCLUSIONS: The inhibiting effect of AcSDKP is not limited to hematoposis cells. AcSDKP is specifically cleaved to an inactive form by the N terminal active site of angiotensin converting enzyme (ACE) and ACE inhibitors (ACEI) can increase the concentration of AcSDKP. It suggests that AcSDKP may be an endogenous modulator of renal cell proliferation and the antiproliferative action of ACEI in renal diseases may be partly mediated by AcSDKP.

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OBJECTIVE: To investigate the effects of malnutrition, nephrosis itself and glucocorticoid therapy on IGF-I/IGFBPs mRNA expressions in the rat liver and kidney, and serum peptides. METHODS: Twenty four male SpragueDawley (SD) rats were randomly grouped into control, pair fed, doxorubincininduced nephrotic and examethasonetreated nephrotic rats. IGF-I/IGFBPs mRNA in the rat liver and kidney, and serum peptides were measured by RT-PCR, RIA and Western ligand blot respectively. RESULTS: ①Reduced serum IGF-I was caused by malnutrition and glucocorticoid therapy rather than nephrosis itself, but IGF-IA mRNA in rat liver and kidney was increased during malnutrition, reduced during glucocorticoid therapy, and unchanged during nephrosis. ②IGFBP-2 mRNA in the rat liver and serum peptides decreased during malnutrition, and were elevated during nephrosis, but serum IGFBP-2 was not changed during glucocorticoid therapy despite its diminished gene expression in the rat liver and kidney. However, IGFBP-2 mRNA in the rat kidney was unchanged during malnutrition, and reduced during nephrosis. ③Serum IGFBP-3 decreased during malnutrition and nephrosis, and increased during glucocorticoid therapy, but reduced IGFBP-3 mRNA in the rat liver was observed during nephrosis and glucocorticoid therapy rather than malnutrition. IGFBP-3 mRNA in the rat kidney was only detectable during glucocorticoid therapy. CONCLUSIONS: The regulation of nutrition, nephrosis and glucocorticoid on IGF-I/IGFBPsd mRNA expressions in the rat liver and kidney is organspecific. The disturbance of serum IGFBPs in the nephrotic syndrome are mainly due to altered liver synthesis except that low serum IGF-I is partly caused by reduced renal synthesis. IGFBPs might regulate the action of IGF-I at different levels in the nephrotic syndrome.

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OBJECTIVE: To investigate the role of nuclear factor kappa B (NF-κB) in the pathogenesis of glomerulonephritis and to determine whether glucocorticoids can inhibit the activation of NF-κB in vivo. METHODS: Nephrotoxic sera nephritis (NTN) was induced by the injection of antiGBM antibody into the tail veins of rats. Glucocorticoidtreated rats received dexamethasone (0.125 mg/kg weight) daily for 14 days. Untreated and steroidtreated rats were killed on day 14 and NF-κB activation and monocyte chemoattractant protein1 (MCP-1) expression were assessed in glomerulus and renal tubules of rats. RESULTS: Significant upregulation of NF-κB activation was observed in glomerulus and renaltubules of untreated NTN rats compared to the control group ［(38.27±8.83)% vs (1.82±0.68)%; (68.46±12.94)% vs (16.89±4.47)%, repsectively］ (P<0.01), and so was the expression of MCP-1 ［(24.37±7.06) cells/gcs vs 0; (54.78±11.49)% vs (11.26±6.88)%］ (P<0.01). NF-κB activation and MCP-1 expression were associated with monocyte cell infiltration and the degree of proteinuria. Significant downregulation of NF-κB activation and MCP-1 expression were observed in the glucocorticoidtreated rats. CONCLUSIONS: The activated NF-κB may play a pivotal pathogenic role in glomerulonephritis and the antinephritic action of glucocorticoids may be mediated through the suppression of the activation of NF-κB.

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OBJECTIVE: To explore the protective effects of nerve growth factor (NGF) on hypoxic-ischemic brain damage (HIBD) in neonatal rats. METHODS: Forty 7day postnatal rats were randomly divided into NGFtreated (n=16), control (n=16) and sham surgery groups (n=8). Immediately after hypoxicischemic (HI) injury, 100 U NGF or normal saline solution was injected intraperitoneally; the sham surgery group was not injected. The effects on body weights, macro and microscopical changes were then assessed. The effect on apoptosis of neurons was observed by terminal deoxynucleotidyl transferase mediated dUTPbiotin nick end labelling (TUNEL) staining. RESULTS: The increased body weight in the NGF treatedgroup was significantly higher than that in the control group ［(4.16±0.24) g and (2.86±0.17) g, respectively (P<0.01)］. The average number of positive cells in the left hippocampus and cortex in the NGFtreated group at 24 h after HIBD were much lower than those in the control group (199.75±19.61 vs 285.50±32.67, 182.75±19.12 vs 271.00±28.36, respectively, P<0.01). At 48 h after HIBD, they were also much lower than those in the control group (77.75±15.76 vs 106.50±16.96; 82.50±19.15 vs 122.75±16.56, respectively, P<0.01). CONCLUSIONS: It is suggested that intraperitoneal administration on NGF has protective effects on neuronal apoptosis associated with hypoxicischemic injury.

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